cd14 rabbit monoclonal antibody Search Results


cd14 (rabbit monoclonal antibody, epr3653  (Biogenex)
90
Biogenex cd14 (rabbit monoclonal antibody, epr3653
(A) ADA2 expression in CD19 + , CD3 + , neutrophils and <t>CD14</t> + immune cell subtypes FACS sorted from peripheral blood or bone marrow of healthy donors detected by immunofluorescence. (B) Analysis of ADA2 expression in different immune cells subtypes analyzed from GSE107011 RNAseq dataset . (C) Volcano plot showing differentially regulated (red=up-regulated, blue=down-regulated) proteins in monocytes proteomes of DADA2 patients in comparison to age and sex-matched healthy donors. (D) ADA2 protein amount, as measured by Label Free Quantification (LFQ intensity) in proteomes from DADA2 patients (left) and healthy controls (right). (E) Pathway analysis showing enrichment of interferon and related pathways in DADA2 proteomes compared to controls. (F) Interaction network on the pathways analysis above. (G) Heatmap showing proteins differentially expressed in DADA2 monocytes with respect to controls. In red are highlighted proteins involved in the interferon response (H) RNA expression of 9 interferon induced genes as measured by realtime PCR in ADA2 -/- and WT THP-1 cells. Heatmap is showing the log2 of the fold change ( ADA2 -/- vs WT ratio). (I) Measurement of CXCL10 concentration in the supernatant of ADA2 -/- and WT THP-1 cells measured by ELISA. The lines over the bars show the statistical comparisons reported in the figure (3 independent experiments, each experiment with duplicates; test used, unpaired two tailed t test with Welch’s correction, *** = p <= 0.001). (J) RNA expression of 8 interferon induced genes as measured by realtime PCR in ADA2 -/- and WT THP-1 cells transduced with lentiviruses carrying human ADA2 or the corresponding empty lentivirus. Y axis shows the log. of the Delta Ct relative to HPRT. The lines over the bars show the statistical comparisons reported in the figure (3 independent experiments, each experiment with duplicates; test used, unpaired two tailed t test with Welch’s correction, ** = p <= 0.001, *** = p <= 0.001, **** = p <= 0.0001).
Cd14 (Rabbit Monoclonal Antibody, Epr3653, supplied by Biogenex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cd14 (rabbit monoclonal antibody, epr3653/product/Biogenex
Average 90 stars, based on 1 article reviews
cd14 (rabbit monoclonal antibody, epr3653 - by Bioz Stars, 2026-04
90/100 stars
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rabbit antihuman cd3  (OriGene)
90
OriGene rabbit antihuman cd3
(A) ADA2 expression in CD19 + , CD3 + , neutrophils and <t>CD14</t> + immune cell subtypes FACS sorted from peripheral blood or bone marrow of healthy donors detected by immunofluorescence. (B) Analysis of ADA2 expression in different immune cells subtypes analyzed from GSE107011 RNAseq dataset . (C) Volcano plot showing differentially regulated (red=up-regulated, blue=down-regulated) proteins in monocytes proteomes of DADA2 patients in comparison to age and sex-matched healthy donors. (D) ADA2 protein amount, as measured by Label Free Quantification (LFQ intensity) in proteomes from DADA2 patients (left) and healthy controls (right). (E) Pathway analysis showing enrichment of interferon and related pathways in DADA2 proteomes compared to controls. (F) Interaction network on the pathways analysis above. (G) Heatmap showing proteins differentially expressed in DADA2 monocytes with respect to controls. In red are highlighted proteins involved in the interferon response (H) RNA expression of 9 interferon induced genes as measured by realtime PCR in ADA2 -/- and WT THP-1 cells. Heatmap is showing the log2 of the fold change ( ADA2 -/- vs WT ratio). (I) Measurement of CXCL10 concentration in the supernatant of ADA2 -/- and WT THP-1 cells measured by ELISA. The lines over the bars show the statistical comparisons reported in the figure (3 independent experiments, each experiment with duplicates; test used, unpaired two tailed t test with Welch’s correction, *** = p <= 0.001). (J) RNA expression of 8 interferon induced genes as measured by realtime PCR in ADA2 -/- and WT THP-1 cells transduced with lentiviruses carrying human ADA2 or the corresponding empty lentivirus. Y axis shows the log. of the Delta Ct relative to HPRT. The lines over the bars show the statistical comparisons reported in the figure (3 independent experiments, each experiment with duplicates; test used, unpaired two tailed t test with Welch’s correction, ** = p <= 0.001, *** = p <= 0.001, **** = p <= 0.0001).
Rabbit Antihuman Cd3, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit antihuman cd3/product/OriGene
Average 90 stars, based on 1 article reviews
rabbit antihuman cd3 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
anti-cd14 rabbit monoclonal antibody  (Boster Bio)
N/A
Boster Bio Anti-CD14 Rabbit Monoclonal Antibody catalog # M00137. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
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cd14 rabbit monoclonal antibody  (Cayman Chemical)
N/A
CD14 RABBIT MONOCLONAL ANTIBODY
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cd14 rabbit monoclonal antibody  (OriGene)
N/A
CD14 rabbit monoclonal antibody clone EPR3653 Supernatant
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Image Search Results


(A) ADA2 expression in CD19 + , CD3 + , neutrophils and CD14 + immune cell subtypes FACS sorted from peripheral blood or bone marrow of healthy donors detected by immunofluorescence. (B) Analysis of ADA2 expression in different immune cells subtypes analyzed from GSE107011 RNAseq dataset . (C) Volcano plot showing differentially regulated (red=up-regulated, blue=down-regulated) proteins in monocytes proteomes of DADA2 patients in comparison to age and sex-matched healthy donors. (D) ADA2 protein amount, as measured by Label Free Quantification (LFQ intensity) in proteomes from DADA2 patients (left) and healthy controls (right). (E) Pathway analysis showing enrichment of interferon and related pathways in DADA2 proteomes compared to controls. (F) Interaction network on the pathways analysis above. (G) Heatmap showing proteins differentially expressed in DADA2 monocytes with respect to controls. In red are highlighted proteins involved in the interferon response (H) RNA expression of 9 interferon induced genes as measured by realtime PCR in ADA2 -/- and WT THP-1 cells. Heatmap is showing the log2 of the fold change ( ADA2 -/- vs WT ratio). (I) Measurement of CXCL10 concentration in the supernatant of ADA2 -/- and WT THP-1 cells measured by ELISA. The lines over the bars show the statistical comparisons reported in the figure (3 independent experiments, each experiment with duplicates; test used, unpaired two tailed t test with Welch’s correction, *** = p <= 0.001). (J) RNA expression of 8 interferon induced genes as measured by realtime PCR in ADA2 -/- and WT THP-1 cells transduced with lentiviruses carrying human ADA2 or the corresponding empty lentivirus. Y axis shows the log. of the Delta Ct relative to HPRT. The lines over the bars show the statistical comparisons reported in the figure (3 independent experiments, each experiment with duplicates; test used, unpaired two tailed t test with Welch’s correction, ** = p <= 0.001, *** = p <= 0.001, **** = p <= 0.0001).

Journal: bioRxiv

Article Title: ADA2 is a lysosomal DNase regulating the type-I interferon response

doi: 10.1101/2020.06.21.162990

Figure Lengend Snippet: (A) ADA2 expression in CD19 + , CD3 + , neutrophils and CD14 + immune cell subtypes FACS sorted from peripheral blood or bone marrow of healthy donors detected by immunofluorescence. (B) Analysis of ADA2 expression in different immune cells subtypes analyzed from GSE107011 RNAseq dataset . (C) Volcano plot showing differentially regulated (red=up-regulated, blue=down-regulated) proteins in monocytes proteomes of DADA2 patients in comparison to age and sex-matched healthy donors. (D) ADA2 protein amount, as measured by Label Free Quantification (LFQ intensity) in proteomes from DADA2 patients (left) and healthy controls (right). (E) Pathway analysis showing enrichment of interferon and related pathways in DADA2 proteomes compared to controls. (F) Interaction network on the pathways analysis above. (G) Heatmap showing proteins differentially expressed in DADA2 monocytes with respect to controls. In red are highlighted proteins involved in the interferon response (H) RNA expression of 9 interferon induced genes as measured by realtime PCR in ADA2 -/- and WT THP-1 cells. Heatmap is showing the log2 of the fold change ( ADA2 -/- vs WT ratio). (I) Measurement of CXCL10 concentration in the supernatant of ADA2 -/- and WT THP-1 cells measured by ELISA. The lines over the bars show the statistical comparisons reported in the figure (3 independent experiments, each experiment with duplicates; test used, unpaired two tailed t test with Welch’s correction, *** = p <= 0.001). (J) RNA expression of 8 interferon induced genes as measured by realtime PCR in ADA2 -/- and WT THP-1 cells transduced with lentiviruses carrying human ADA2 or the corresponding empty lentivirus. Y axis shows the log. of the Delta Ct relative to HPRT. The lines over the bars show the statistical comparisons reported in the figure (3 independent experiments, each experiment with duplicates; test used, unpaired two tailed t test with Welch’s correction, ** = p <= 0.001, *** = p <= 0.001, **** = p <= 0.0001).

Article Snippet: Staining was done with a primary antibody against CD14 (rabbit monoclonal antibody, clone EPR3653, BioGenex, Fremont, USA), no antigen retrieval was necessary.

Techniques: Expressing, Immunofluorescence, RNA Expression, Concentration Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Transduction

(A-E) Lymph node; (A) Few follicles (pointed by arrowhead) withou germinal centers. (B) CD3 staining displays normally distributed T-cells (arrowhead points to B-cell follicle with lower density of CD3+ T-cells). (C) Lack of IgG+ plasma cells. Serum is IgG positive due to replacement therapy. The expanded stroma of the lymph node is highlighted by asterisk. (D) Severely ectatic subcapsular sinus (dotted line) with multiple macrophages, most of them foam cells histiocytosis. (E) CD68 displays the multiple macrophages of the sinus F) Spleen: Intact periarteriolar lymphocytic sheath (pointed by arrowhead) next to an infarction (kollagenous scar tissue marked by asterisk, fibrin marked by double asterisk). (G-H) Immunohistochemistry of human tonsil showing CD14 (red) and ADA2 (blue); dotted line show germinal centers with cytoplasmic ADA2 positivity (asterisk, exemplary) in tingible body macrophages; CD14 (red) highlights the follicular dendritic cell meshwork (10x original magnification). In higher magnifications (bottom panels), CD14 + dendritic cells in the crypt epithelium (left panel) and surface epithelium (right panel) show few small ADA2 positive intracytoplasmic granules (highlighted by arrowheads, exemplary; 20x original magnification).

Journal: bioRxiv

Article Title: ADA2 is a lysosomal DNase regulating the type-I interferon response

doi: 10.1101/2020.06.21.162990

Figure Lengend Snippet: (A-E) Lymph node; (A) Few follicles (pointed by arrowhead) withou germinal centers. (B) CD3 staining displays normally distributed T-cells (arrowhead points to B-cell follicle with lower density of CD3+ T-cells). (C) Lack of IgG+ plasma cells. Serum is IgG positive due to replacement therapy. The expanded stroma of the lymph node is highlighted by asterisk. (D) Severely ectatic subcapsular sinus (dotted line) with multiple macrophages, most of them foam cells histiocytosis. (E) CD68 displays the multiple macrophages of the sinus F) Spleen: Intact periarteriolar lymphocytic sheath (pointed by arrowhead) next to an infarction (kollagenous scar tissue marked by asterisk, fibrin marked by double asterisk). (G-H) Immunohistochemistry of human tonsil showing CD14 (red) and ADA2 (blue); dotted line show germinal centers with cytoplasmic ADA2 positivity (asterisk, exemplary) in tingible body macrophages; CD14 (red) highlights the follicular dendritic cell meshwork (10x original magnification). In higher magnifications (bottom panels), CD14 + dendritic cells in the crypt epithelium (left panel) and surface epithelium (right panel) show few small ADA2 positive intracytoplasmic granules (highlighted by arrowheads, exemplary; 20x original magnification).

Article Snippet: Staining was done with a primary antibody against CD14 (rabbit monoclonal antibody, clone EPR3653, BioGenex, Fremont, USA), no antigen retrieval was necessary.

Techniques: Staining, Immunohistochemistry